2017-8-11 16:0:32025-05-23 06:42:46
locus
BSU23870
BSU_23870
geneLength
1242
1245
outlinks
bsu
BSU23870
BSU_23870
Gene
Phenotypes of a mutant
reduced UV-induced mutagenesis [Pubmed|12644484]
reduced UV-induced mutagenesis [Pubmed|12644484]
sensitive to blue light-induced DNA damage [pubmed|30054368]
increased sensitivity to Cr(VI) [pubmed|30745368]
The protein
Catalyzed reaction/ biological activity
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for Cr(VI)-induced mutagenesis [pubmed|30745368]
The protein
Protein family
DNA polymerase type-Y family (according to Swiss-Prot)
[SW|DNA polymerase type-Y family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|PolY2]], [[protein|UvrX]], [[protein|YozK]]-[[protein|YobH]]
[[this]]
Biological materials
Mutant
MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]
GP1111 (spc), available in [SW|Stülke] lab
MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]
GP1111 (spc), available in [SW|Jörg Stülke]'s lab [pubmed|31948638]
BKE23870 (Δ[[gene|polY1]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
BKK23870 (Δ[[gene|polY1]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
References
Reviews
References
Original publications
The protein
[SW|Domains]
[SW|UmuC domain] (aa 8-189) (according to UniProt)
The protein
Structure
[PDB|4IRC] (from E. coli, 35% identity) [pubmed|23525461]