SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-23 06:42:46

locus

BSU23870

BSU_23870

geneLength

1242

1245

outlinks

bsu

BSU23870

BSU_23870

Gene

Phenotypes of a mutant

reduced UV-induced mutagenesis [Pubmed|12644484]

reduced UV-induced mutagenesis [Pubmed|12644484]

sensitive to blue light-induced DNA damage [pubmed|30054368]

increased sensitivity to Cr(VI) [pubmed|30745368]

The protein

Catalyzed reaction/ biological activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)

required for Cr(VI)-induced mutagenesis [pubmed|30745368]

The protein

Protein family

DNA polymerase type-Y family (according to Swiss-Prot)

[SW|DNA polymerase type-Y family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|PolY2]], [[protein|UvrX]], [[protein|YozK]]-[[protein|YobH]]

[[this]]

Biological materials

Mutant

MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]

GP1111 (spc), available in [SW|Stülke] lab

MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]

GP1111 (spc), available in [SW|Jörg Stülke]'s lab [pubmed|31948638]

BKE23870 (Δ[[gene|polY1]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG

BKK23870 (Δ[[gene|polY1]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG

References

Reviews

22933559

22933559, 29856930

References

Original publications

12644484, 15469515, 16045613, 19924481, 23686288, 25713353

12644484, 15469515, 16045613, 19924481, 23686288, 25713353, 30054368, 30096406, 23525461, 30745368, 30916324, 31948638

The protein

[SW|Domains]

[SW|UmuC domain] (aa 8-189) (according to UniProt)

The protein

Structure

[PDB|4IRC] (from E. coli, 35% identity) [pubmed|23525461]